Affiliation:
1. Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain
2. Department of Functional Biology, Immunology Area, University of Oviedo, Asturias, Spain
Abstract
ABSTRACT
Probiotics play an important role in the maintenance of the gastrointestinal barrier. In addition to direct effects on mucosal integrity, the interaction with the intestinal mucosa may have an active immunoregulatory effect. In the present work, we exposed HT29 intestinal epithelial cells to two
Bifidobacterium
species to determine their effect on gene expression profile, enterocyte monolayer integrity, and T-cell response.
Bifidobacterium breve
IPLA 20004 triggered a more pronounced increase in the transepithelial resistance of the enterocyte monolayer than
Bifidobacterium bifidum
LMG13195. The transcriptome profile of HT29 cells cultured in the presence of
B. bifidum
LMG13195 showed an increased expression of immune mediators and, interestingly, chemotactic molecules (CXCL10, CCL20, CXCL11 and CCL22) able to recruit lymphocytes. Since regulatory T cells (Treg cells) may express receptors for specific chemokines, we cultured peripheral blood mononuclear cells with supernatants of HT29 cells previously treated with
Bifidobacterium
strains and analyzed FOXP3 and CD25 Treg markers and CCR6, CXCR3, CCR4, and CCR3 expression on CD4
+
lymphocytes. The proportion of CD25
high
FOXP3
+
cells was significantly increased after culture with
B. bifidum
LMG13195-conditioned HT29 supernatant. Moreover, this treatment led to the largest amount of CCR6
+
CXCR3
−
CCR4
+
CCR3
+
CD4
+
cells expressing high levels of CD25, corresponding to the Treg population. These results suggest that soluble factors secreted after
B. bifidum
LMG13195 contact with intestinal epithelial cells favored the generation of CD4
+
CD25
high
lymphocytes expressing chemokine receptor Treg markers, thus making possible their recruitment to the intestinal mucosa.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
50 articles.
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