Affiliation:
1. National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta 700010
2. Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri
3. International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
4. Institute of Post Graduate Medical Education and Research, Calcutta 700020, India
Abstract
ABSTRACT
Virulence-associated genes and neutral DNA markers of
Helicobacter pylori
strains from the Santhal and Oroan ethnic minorities of West Bengal, India, were studied. These people have traditionally been quite separate from other Indians and differ culturally, genetically, and linguistically from mainstream Bengalis, whose
H. pylori
strains have been characterized previously.
H. pylori
was found in each of 49 study participants, although none had peptic ulcer disease, and was cultured from 31 of them. All strains carried the
cag
pathogenicity island and potentially toxigenic s1 alleles of vacuolating cytotoxin gene (
vacA
) and were resistant to at least 8 μg of metronidazole per ml. DNA sequence motifs in
vacA
mid-region m1 alleles,
cagA
, and an informative insertion or deletion motif next to
cagA
from these strains were similar to those of strains from ethnic Bengalis. Three mobile elements, IS
605
, IS
607
, and IS
Hp608
, were present in 29, 19, and 10%, respectively, of Santhal and Oroan strains, which is similar to their prevalence in Bengali
H. pylori.
Thus, there is no evidence that the gene pools of
H. pylori
of these ethnic minorities differ from those of Bengalis from the same region. This relatedness of strains from persons of different ethnicities bears on our understanding of
H. pylori
transmission between communities and genome evolution.
Publisher
American Society for Microbiology
Reference47 articles.
1. Achtman, M., T. Azuma, D. E. Berg, Y. Ito, G. Morelli, Z. J. Pan, S. Suerbaum, S. A. Thompson, A. van der Ende, and L. J. van Doorn. 1999. Recombination and clonal groupings within Helicobacter pylori from different geographical regions. Mol. Microbiol.32:459-470.
2. Akopyanz, N., N. O. Bukanov, T. U. Westblom, S. Kresovich, and D. E. Berg. 1992. DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res.20:5137-5142.
3. Akopyants, N. S., S. W. Clifton, D. Kersulyte, J. E. Crabtree, B. E. Youree, C. A. Reece, N. O. Bukanov, E. S. Drazek, B. A. Roe, and D. E. Berg. 1998. Analyses of the cag pathogenicity island of Helicobacter pylori.Mol. Microbiol.28:37-54.
4. Anonymous. 1986. Rapid identification of pyloric Campylobacter in Peruvians with gastritis. Dig. Dis. Sci.31:1089-1094.
5. Blaser, M. J. 1998. Helicobacters are indigenous to the human stomach: duodenal ulceration is due to changes in gastric microecology in the modern era. Gut43:721-727.
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