Affiliation:
1. Departments of Biochemistry 1 and
2. Pathology and Cell Biology, 2 University of Montreal, Montreal, Quebec, Canada H3C 3J7
Abstract
ABSTRACT
Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization in
Drosophila.
To understand the molecular mechanisms of mRNA transport in mammals, we cloned human (
hStau
) and mouse (
mStau
)
staufen
cDNAs. In humans, four transcripts arise by differential splicing of the
Stau
gene and code for two proteins with different N-terminal extremities. In vitro, hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and tubulin via a region similar to the microtubule-binding domain of MAP-1B, suggesting that Stau cross-links cytoskeletal and RNA components. Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process involving microtubules. These results are the first demonstration of the association of an RNA-binding protein in addition to ribosomal proteins, with the RER, implicating this class of proteins in the transport of RNA to its site of translation.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
194 articles.
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