PCR Amplification with Primers Based on IS 2404 and GC-Rich Repeated Sequence Reveals Polymorphism in Mycobacterium ulcerans-Reference-Cited by-同舟云学术

PCR Amplification with Primers Based on IS 2404 and GC-Rich Repeated Sequence Reveals Polymorphism in Mycobacterium ulcerans

Published:2005-01 Issue:1 Volume:43 Page:448-451
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ablordey Anthony¹,Kotlowski Roman¹²,Swings Jean³,Portaels Françoise¹

Affiliation:

1. Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp

2. Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland

3. Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium

Abstract

ABSTRACT We describe a simple genotyping method for Mycobacterium ulcerans based on PCR amplification of genomic regions between IS 2404 and a frequently repeated GC-rich sequence. Application of this method to a global collection produced 10 M. ulcerans genotypes corresponding to their geographic origin.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.1.448-451.2005

Reference11 articles.

1. Asiedu K. R. Scherpbier and M. Raviglione. 2000. Buruli ulcer infection: Mycobacterium ulcerans infection p. 1-4. (W.H.O./CDS/CPE/GBUI/2000.1) World Health Organization Geneva Switzerland.

2. Evaluation of PCR-Restriction Profile Analysis and IS 2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans and M. marinum

3. Chemlal, K., K. de Ridder, P. A. Fonteyne, W. M. Meyers, J. Swings, and F. Portaels. 2001. The use of IS2404 restriction fragment length polymorphisms suggests the diversity of Mycobacterium ulcerans from different geographic areas. Am. J. Trop. Med. Hyg.64:270-273.

4. Characterization of an Unusual Mycobacterium: a Possible Missing Link between Mycobacterium marinum and Mycobacterium ulcerans

5. PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS 6110 Inverted Repeats Used as Primers

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