PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS 6110 Inverted Repeats Used as Primers-Reference-Cited by-同舟云学术

PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS 6110 Inverted Repeats Used as Primers

Published:2004-01 Issue:1 Volume:42 Page:372-377
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kotłowski Roman¹,Shamputa Isdore Chola¹,El Aila Nabil Abdullah¹,Sajduda Anna¹,Rigouts Leen¹,van Deun Armand¹,Portaels Françoise¹

Affiliation:

1. Mycobacteriology Unit, Prince Leopold Institute of Tropical Medicine, B-2000 Antwerp, Belgium

Abstract

ABSTRACT In the present study we attempted to develop a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis isolates. Use of the designed primers Mtb1 (5′-CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5′-CGG-CGG-CAA-CGG-CGG-C) targeting frequently repeated 16-bp sequences in combination with primers sited at the inverted repeats flanking IS 6110 allowed differentiation of M. tuberculosis isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.1.372-377.2004

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