Affiliation:
1. Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen
2. The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford, United Kingdom
Abstract
ABSTRACT
Two new species,
Candida orthopsilosis
and
C. metapsilosis
, are proposed to replace the existing designations of
C. parapsilosis
groups II and III, respectively. The species
C. parapsilosis
is retained for group I isolates. Attempts to construct a multilocus sequence typing scheme to differentiate individual strains of
C. parapsilosis
instead revealed fixed DNA sequence differences between pairs of subgroups in four genes:
COX3
,
L1A1
,
SADH
, and
SYA1
. PCR amplicons for sequencing were obtained for these four plus a further seven genes from 21 group I isolates. For nine group II isolates, PCR products were obtained from only 5 of the 11 genes, and for two group III isolates PCR products were obtained from a different set of 5 genes. Three of the PCR products from group II and III isolates differed in size from the group I products. Cluster analysis of sequence polymorphisms from
COX3
,
SADH
, and
SYA1
, which were common to the three groups, consistently separated the isolates into three distinct sets. All of these differences, together with DNA sequence similarities <90% in the
ITS1
sequence, suggest the subgroups should be afforded species status. The near absence of DNA sequence variability among isolates of
C. parapsilosis
and relatively high levels of sequence variability among isolates of
C. orthopsilosis
suggest that the former species may have evolved very recently from the latter.
Publisher
American Society for Microbiology
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