Collaborative Consensus for Optimized Multilocus Sequence Typing of Candida albicans

Author:

Bougnoux M.-E.12,Tavanti A.3,Bouchier C.4,Gow N. A. R.3,Magnier A.4,Davidson A. D.3,Maiden M. C. J.5,d'Enfert C.1,Odds F. C.3

Affiliation:

1. Unité Biologie et Pathogénicité Fongiques, CNRS URA 2172

2. Laboratoire de Parasitologie-Mycologie, Service de Microbiologie, Hôpital Ambroise-Paré, Université Versailles Saint-Quentin en Yvelines, Boulogne-Billancourt, France

3. Department of Molecular & Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen

4. Génopole, Institut Pasteur, Paris

5. The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford, United Kingdom

Abstract

ABSTRACT A panel of 86 different Candida albicans isolates was subjected to multilocus sequence typing (MLST) in two laboratories to obtain sequence data for 10 published housekeeping gene fragments. Analysis of data for all possible combinations of five, six, seven, eight, and nine of the fragments showed that a set comprising the fragments AAT1a , ACC1 , ADP1 , MPIb , SYA1 , VPS13 , and ZWF1b was the smallest that yielded 86 unique diploid sequence types for the 86 isolates. This set is recommended for future MLST with C. albicans .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference6 articles.

1. Usefulness of Multilocus Sequence Typing for Characterization of Clinical Isolates of Candida albicans

2. Multilocus Sequence Typing System for Group B Streptococcus

3. Magee P. T. and H. Chibana. 2002. The genomes of Candida albicans and other Candida species. In R. A. Calderone (ed.) Candida and candidiasis. ASM Press Washington D.C.

4. Nei, M., and T. Gojobori. 1986. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol. Biol. Evol.3:418-426.

5. Optimization and Validation of Multilocus Sequence Typing for Candida albicans

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