Virus Passage through Track-Etch Membranes Modified by Salinity and a Nonionic Surfactant

Author:

Lytle C. David1,Routson Licia B.1,Jain Nisha B.2,Myers Matthew R.1,Green Barbara L.3

Affiliation:

1. Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland 208571;

2. Thomas Jefferson High School for Science and Technology, Alexandria, Virginia 223122; and

3. Corning, Incorporated, Acton, Massachusetts 017203

Abstract

ABSTRACT Why do viruses sometimes not pass through larger pores in track-etch filters? Increasing the salinity (0.8 to 160 mM Na + ) decreased φX174 and PRD1 passage through track-etch polycarbonate membranes (sodium dodecyl sulfate coated but not polyvinylpyrrolidone coated) and PRD1 passage through polyester membranes. Undiminished passage when 0.1% Tween 80 was added implied that nonionic virus adsorption occurred and indicated that high levels of salinity decreased virus passage by decreasing electrostatic repulsion that prevented adsorption.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference13 articles.

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2. Brinton C. Lauffer M. The electrophoresis of viruses bacteria and cells and the microscope method of electrophoresis Electrophoresis. Bier M. 1959 427 492 Academic Press Inc. New York N.Y

3. Francki R. I. B. C. M. Fauquet D. L. Knudson and F. Brown. 1991. Classification and nomenclature of viruses. Arch. Virol. Suppl. 2 .

4. Elution of viruses by ionic and nonionic surfactants

5. Determining the size of non-A, non-B hepatitis virus by filtration.;He L.-F.;J. Infect. Dis.,1987

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