Elution of viruses by ionic and nonionic surfactants

Author:

Fujito B T1,Lytle C D1

Affiliation:

1. Thomas Jefferson High School for Science and Technology, Alexandria, Virginia 22312, USA.

Abstract

The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when combinations of surfactants and binding membranes were matched (i.e., ionic-ionic or nonionic-nonionic).

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference17 articles.

1. Ackermann H.-W. 1987. Cubic filamentous and pleomorphic phages p. 171-218. In H. W. Ackermann and M. S. DuBow (ed.) Viruses in prokaryotes vol. II. CRC Press Inc. Boca Raton Fla.

2. American Society for Testing and Materials. 1995. Standard test method for resistance of materials used in protective clothing to penetration by bloodborne pathogens using viral penetration as a test system. Standard F16 71-95. American Society for Testing and Materials Philadelphia.

3. Chemical factors influencing adsorption of bacteriophage MS2 to membrane filters;Farrah S. R.;Appl. Environ. Microbiol.,1982

4. Effects of chaotropic and antichaotropic agents on elution of poliovirus adsorbed on membrane filters;Farrah S. R.;Proc. Natl. Acad. Sci. USA,1981

5. Properties of detergents;Helenius A.;Methods Enzymol.,1979

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