Affiliation:
1. Department of Bacteriology and Immunology, University of Western Ontario, London, Ontario, Canada
Abstract
A stage 4 sporulation mutant of a strain of
Bacillus cereus
var.
alesti
fails to synthesize a cortex although all other structural components appear normal. With terminal lysis the spore core as well as the sporangium is lysed. Both the uptake of
45
Ca and the synthesis of dipicolinic acid (DPA) are similar to these activities in the parent strain, but these components (DPA and Ca) are lost to the medium with the drastic lysis. The first stage of diaminopimelic acid incorporation, that into germ cell wall mucopeptide, is intact in the mutant; the second stage, that into cortical mucopeptide, is absent. These biochemical studies as well as phospholipid metabolism and freeze-etch analysis suggest the lesion lies in the outer forespore membrane.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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