The cortical peptidoglycan from spores of Bacillus megaterium and Bacillus subtilis is not highly cross-linked

Author:

Popham D L1,Setlow P1

Affiliation:

1. Department of Biochemistry, University of Connecticut Health Center, Farmington 06030-3305.

Abstract

Determination by amino acid analyses of the percentage of diaminopimelic acid in the spore cortex of Bacillus megaterium and Bacillus subtilis which is involved in interpeptide cross-links gave values of 31 to 37%. This finding supports the idea that the cortex volume could undergo significant changes in response to changes in pH or ionic strength and could thus play an active role in reducing the water content of the spore protoplast during sporulation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference15 articles.

1. Mutagenesis and mapping of the gene for a sporulation-specific penicillin-binding protein in Bacillus subtilis;Buchanan C. E.;J. Bacteriol.,1992

2. Purification and amino acid sequence of two small, acid-soluble proteins from Clostridium bifermentans spores;Cabrera-Martinez R.;FEMS Microbiol. Lett.,1989

3. Characterization of cross-linking of cell walls ofBacillus subtilis by a combination of magic-angle spinning NMR and gas chromatography-mass spectrometry of both intact and hydrolyzed 13C- and 15N-labeled cell-wall peptidoglycan;Forrest T. M.;J. Biol. Chem.,1991

4. Gerhardt P. and R. E. Marquis. 1989. Spore thermoresistance mechanisms p. 43-63. In I. Smith R. A. Slepecky and P. Setlow (ed.) Regulation of procaryotic development. American Society for Microbiology Washington D.C.

5. Relationship between cortex content and properties of Bacillus sphaericus spores;Imae Y.;J. Bacteriol.,1976

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