Affiliation:
1. Department of Animal Science, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691
Abstract
The carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. Increasing concentrations of CO
2
were added as NaHCO
3
to a specially prepared CO
2
-free medium which was tubed and inoculated under nitrogen. Prior depletion of CO
2
in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. An absolute requirement for CO
2
was observed for eight strains of
Bacteroides ruminicola
, three strains of
Bacteroides succinogenes
, four strains of
Ruminococcus flavefaciens
, two strains of
Lachnospira multiparus
, one strain of
Succinimonas amylolytica
, and two strains of
Butyrivibrio fibrisolvens
. Inconsistent growth responses were obtained in CO
2
-free media with one strain each of
B. fibrisolvens, Ruminococcus albus
, and
Selenomonas ruminantium
. Growth of six additional strains of
B. fibrisolvens
, and single strains of
Eubacterium ruminantium
and
Succinivibrio dextrinosolvens
was markedly increased or stimulated by increasing concentrations of CO
2
.
Peptostreptococcus elsdenii
B159 was the only organism tested which appeared to have no requirement, either absolute or partial, for CO
2
. Higher concentrations of CO
2
were required for the initiation of growth, as well as for optimal growth, by those species which produce succinic acid as one of their primary end products.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference22 articles.
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