Enzymatic Synthesis of Novel Phloretin Glucosides-Reference-Cited by-同舟云学术

Enzymatic Synthesis of Novel Phloretin Glucosides

Published:2013-06 Issue:11 Volume:79 Page:3516-3521
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Pandey Ramesh Prasad¹,Li Tai Feng¹,Kim Eun-Hee²,Yamaguchi Tokutaro¹,Park Yong Il³,Kim Joong Su⁴,Sohng Jae Kyung¹

Affiliation:

1. Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, Tangjeong-myeon, Asansi, Chungnam, Republic of Korea

2. Division of Magnetic Resonance, Korea Basic Science Institute, Ochang, Chungbuk, Republic of Korea

3. Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Republic of Korea

4. Bioindustry Process Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk, Jeong-Ub, Republic of Korea

Abstract

ABSTRACT A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00409-13

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