Inhibition of PCR by Agar from Bacteriological Transport Media-Reference-Cited by-同舟云学术

Inhibition of PCR by Agar from Bacteriological Transport Media

Published:1998-01 Issue:1 Volume:36 Page:275-276
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gibb Alan Patrick¹²³,Wong Sallene¹

Affiliation:

1. Provincial Laboratory for Public Health of Southern Alberta,1

2. Department of Microbiology and Infectious Diseases, University of Calgary,2 and

3. Department of Microbiology and Infectious Diseases, Foothills Hospital,3Calgary, Alberta, Canada

Abstract

ABSTRACT We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.1.275-276.1998

Reference7 articles.

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2. Inhibition of the polymerase chain reaction by mucolytic agents;Deneer H. G.;Clin. Chem.,1994

3. Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates

4. Comparison of nasopharyngeal aspirates and throat swab specimens in a polymerase chain reaction-based test for Mycoplasma pneumoniae;Reznikov M.;Eur. J. Clin. Microbiol. Infect. Dis.,1995

5. Detection of Mycoplasma pneumoniae in clinical samples from pediatric patients by polymerase chain reaction

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