Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories-Reference-Cited by-同舟云学术

Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

Published:2020-09-30 Issue:6 Volume:32 Page:815-825
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Toohey-Kurth Kathy L.¹^ORCID,Mulrooney Donna M.²,Hinkley Susanne³,Lea Killian Mary⁴,Pedersen Janice C.⁴,Bounpheng Mangkey A.⁵,Pogranichniy Roman⁶,Bolin Steve⁷,Maes Roger⁷,Tallmadge Rebecca L.⁸^ORCID,Goodman Laura B.⁸^ORCID,Crossley Beate M.⁹^ORCID

Affiliation:

1. California Animal Health and Food Safety Laboratory, University of California–Davis, San Bernardino

2. Oregon Veterinary Diagnostic Laboratory, Oregon State University, Corvallis, OR

3. Neogen Corporation, Lincoln, NE

4. National Veterinary Services Laboratories, Ames, IA

5. Texas A&M University, College Station, TX

6. Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS

7. Veterinary Diagnostic Laboratory, Michigan State University, Lansing, MI

8. Population Medicine & Diagnostic Sciences, Cornell University, Ithaca, NY

9. California Animal Health and Food Safety Laboratory, University of California–Davis, San Bernardino and Davis branches, CA

Abstract

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/1040638720962076

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