Affiliation:
1. Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, Connecticut
Abstract
ABSTRACT
We have identified
gas5
(growth arrest-specific transcript 5) as a non-protein-coding multiple small nucleolar RNA (snoRNA) host gene similar to
UHG
(U22 host gene). Encoded within the 11 introns of the mouse
gas5
gene are nine (10 in human) box C/D snoRNAs predicted to function in the 2′-O-methylation of rRNA. The only regions of conservation between mouse and human
gas5
genes are their snoRNAs and 5′-end sequences. Mapping the 5′ end of the mouse
gas5
transcript demonstrates that it possesses an oligopyrimidine tract characteristic of the 5′-terminal oligopyrimidine (5′TOP) class of genes. Arrest of cell growth or inhibition of translation by cycloheximide, pactamycin, or rapamycin—which specifically inhibits the translation of 5′TOP mRNAs—results in accumulation of the
gas5
spliced RNA. Classification of
gas5
as a 5′TOP gene provides an explanation for why it is a growth arrest specific transcript: while the spliced
gas5
RNA is normally associated with ribosomes and rapidly degraded, during arrested cell growth it accumulates in mRNP particles, as has been reported for other 5′TOP messages. Strikingly, inspection of the 5′-end sequences of currently known snoRNA host gene transcripts reveals that they all exhibit features of the 5′TOP gene family.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
385 articles.
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