Effects of Environmental Stress on Stability of Tandem Repeats in Escherichia coli O157:H7

Author:

Cooley Michael B.1,Carychao Diana1,Nguyen Kimberly1,Whitehand Linda2,Mandrell Robert1

Affiliation:

1. Produce Safety and Microbiology Research Unit

2. Western Regional Research Center, USDA Agricultural Research Service, 800 Buchanan St., Albany, California 94710

Abstract

ABSTRACT Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication but changed after exposure to irradiation, elevated temperatures, and starvation conditions. The pO157 plasmid was frequently lost under these stress conditions. Environmental stresses may increase phylogenetic diversity as measured by MLVA.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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