Zebrafish as a Model Host for Candida albicans Infection

Author:

Chao Chun-Cheih1,Hsu Po-Chen2,Jen Chung-Feng1,Chen I-Hui1,Wang Chieh-Huei1,Chan Hau-Chien1,Tsai Pei-Wen2,Tung Kai-Che1,Wang Chian-Huei1,Lan Chung-Yu23,Chuang Yung-Jen13

Affiliation:

1. Institute of Bioinformatics and Structure Biology

2. Institute of Molecular and Cellular Biology

3. Department of Life Science, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan, Republic of China

Abstract

ABSTRACT In this work, the zebrafish model organism was developed to obtain a minivertebrate host system for a Candida albicans infection study. We demonstrated that C. albicans can colonize and invade zebrafish at multiple anatomical sites and kill the fish in a dose-dependent manner. Inside zebrafish, we monitored the progression of the C. albicans yeast-to-hypha transition by tracking morphogenesis, and we monitored the corresponding gene expression of the pathogen and the early host immune response. We performed a zebrafish survival assay with different C. albicans strains (SC5314, ATCC 10231, an hgc1 mutant, and a cph1 / efg1 double mutant) to determine each strain's virulence, and the results were similar to findings reported in previous mouse model studies. Finally, using zebrafish embryos, we monitored C. albicans infection and visualized the interaction between pathogen and host myelomonocytic cells in vivo . Taken together, the results of this work demonstrate that zebrafish can be a useful host model to study C. albicans pathogenesis, and they highlight the advantages of using the zebrafish model in future invasive fungal research.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

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