Whole-Genome Sequences of Antibiotic-Resistant Trueperella pyogenes Isolates from Surgical Site Infections in Dairy Cows in Switzerland

Author:

Marchionatti Emma12ORCID,Perreten Vincent2ORCID

Affiliation:

1. Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland

2. Division of Molecular Bacterial Epidemiology and Infectious Diseases, Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland

Abstract

The complete genome sequence of four Trueperella pyogenes isolated from cattle surgical site infections in Switzerland was determined using hybrid assembly of Oxford Nanopore and Illumina reads. Genes conferring resistance to tetracyclines [ tet (W)], sulfonamides ( sul1 ), chloramphenicol ( cmx ), streptomycin/spectinomycin ( aadA1 ), and quaternary ammonium compounds ( qacE Δ 1 ) were identified on different chromosomal elements.

Funder

University of Bern

Bundesamt für Lebensmittelsicherheit und Veterinärwesen

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Reference11 articles.

1. Pathogenicity and Virulence of Trueperella pyogenes: A Review

2. Antimicrobial Resistance in Corynebacterium spp., Arcanobacterium spp., and Trueperella pyogenes

3. CLSI. 2017. Methods for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria isolated from animals, 1st ed. CLSI supplement VET06. Clinical and Laboratory Standards Institute, Wayne, PA.

4. Trimmomatic: a flexible trimmer for Illumina sequence data

5. Performance of neural network basecalling tools for Oxford Nanopore sequencing

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