Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs-Reference-Cited by-同舟云学术

Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs

Published:1990-06 Issue:6 Volume:34 Page:1075-1078
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Merali S¹,Zhang Y¹,Sloan D¹,Meshnick S¹

Affiliation:

1. Department of Chemistry, City University of New York Medical School, City College of City University of New York, New York 10031.

Abstract

A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 14 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.34.6.1075

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