Avian Salmonella -Stained Microtest Antigens Produced on Solid Media

Author:

Williams J. E.1,Whittemore A. D.1

Affiliation:

1. Southeast Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30601

Abstract

Procedures are described for the preparation of Salmonella pullorum and Salmonella typhimurium tetrazolium-stained microagglutination and microantiglobulin antigens with solid media for culture propagation. Cell suspensions were found to be more easily harvested from solid medium than from previously used liquid medium, and greater yields of antigen were obtained. Liquid medium microagglutination and microantiglobulin test antigens were found to be more sensitive than those prepared on solid medium. The microtest antigens produced on solid medium were easier to prepare and in serological tests gave titers more comparable to those demonstrated with the standard macroscopic tube agglutination test.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference4 articles.

1. Anonymous. 1971. Methods for examining poultry biologics and for identifying and quantifying avian pathogens chapter 13. Nat. Acad. Sci. Nat. Res. Counc. Publ. No. ISBN 0-309-01853-6 Washington D.C.

2. Micro-agglutination test for toxoplasma antibodies;Fulton J. D.;Immunology (Brit.),1965

3. Serological diagnosis of pullorum disease with the microagglutination system;Williams J. E.;Appl. Microbiol.,1971

4. Microantiglobulin test for detecting Salmonella typhimurium agglutinins;Williams J. E.;Appl. Microbiol.,1972

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