Microantiglobulin Test for Detecting Salmonella typhimurium Agglutinins

Author:

Williams J. E.1,Whittemore A. D.1

Affiliation:

1. Southeast Poultry Research Laboratory, Veterinary Sciences Research Division, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30601

Abstract

A sensitive antiglobulin (AG) test procedure for the demonstration and experimental study of the agglutinin response of chickens infected orally with Salmonella typhimurium is described. A tetrazolium-stained S. typhimurium antigen was employed with microagglutination techniques and equipment for the first time in conducting the AG test. Results with the conventional macroscopy tube agglutination test for S. typhimurium and the 24-hr microtest were comparable; however, the AG test enhanced titers as much as 16 times, and these persisted at a significant level for as long as 4 months. This study is being extended to other Salmonella serotypes and possible field applications of the AG test procedure.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference21 articles.

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3. Anonymous. 1971. The National poultry improvement plan and auxiliary provisions. Animal Science Research Division Agricultural Research Service USDA Beltsville Md.

4. Anonymous. 1971. Methods for examining poultry biologics and for identifying and quantifying avian pathogens. Nat. Acad. Sci. Nat. Res. Counc. Publ. no. ISBN 0-309-01853-6 Washington D.C.

5. Avian salmonellosis in England and Wales 1948-56 with comment on its prevention and control;Blaxland J. D.;Vet. Rec.,1958

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