Phenotypic and genotypic biotyping of environmental strains of Vibrio cholerae non-O1 isolated in Italy

Author:

Filetici E1,Bonadonna L1,Ciccozzi M1,Anastasio M P1,Fantasia M1,Shimada T1

Affiliation:

1. Istituto Superiore di Sanità, Rome, Italy.

Abstract

The purpose of this study was to characterize strains of Vibrio cholerae non-O1 isolated in Italy from different sources by biochemical and serological assays, antibiotic susceptibility testing, and molecular biotyping. Serotyping and genomic analysis by pulsed-field gel electrophoresis proved to be useful in discriminating the isolates. The data obtained show a wide heterogeneity at the genomic level, and in keeping with this, the serogrouping classification provided evidence of a high variability of the investigated strains. In addition, none of the strains tested produced cholera-like toxins.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference42 articles.

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