Simultaneous Directed Evolution of Coupled Enzymes for Efficient Asymmetric Synthesis of l -Phosphinothricin-Reference-Cited by-同舟云学术

Simultaneous Directed Evolution of Coupled Enzymes for Efficient Asymmetric Synthesis of l -Phosphinothricin

Published:2021-02-12 Issue:5 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cheng Feng¹²,Li Qing-Hua¹²,Zhang Hua-Yue¹²,Wei Lan¹²,Zhang Jia-Min¹²,Li Ju-Mou¹²,Xue Ya-Ping¹²^ORCID,Zheng Yu-Guo¹²

Affiliation:

1. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, People’s Republic of China

2. The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou, People’s Republic of China

Abstract

The traditional strategy to improve multienzyme-catalyzed reaction efficiencies may lead to enhanced single-enzyme catalytic efficiencies but may also result in the loss of coordination among enzymes. We describe a directed evolution strategy for an entire coupled-enzyme system to simultaneously enhance enzyme coordination and catalytic efficiency.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02563-20

Reference48 articles.

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2. Artificial Biocatalytic Linear Cascades for Preparation of Organic Molecules

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