Inefficiency of 0.3% Carbol Fuchsin in Ziehl-Neelsen Staining for Detecting Acid-Fast Bacilli-Reference-Cited by-同舟云学术

Inefficiency of 0.3% Carbol Fuchsin in Ziehl-Neelsen Staining for Detecting Acid-Fast Bacilli

Published:2002-08 Issue:8 Volume:40 Page:3041-3043
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Selvakumar N.¹,Rahman Fathima¹,Rajasekaran S.²,Narayanan P. R.¹,Frieden Thomas R.³

Affiliation:

1. Tuberculosis Research Centre, Indian Council of Medical Research, Chennai, 600 031 Tamil Nadu

2. Government Hospital for Thoracic Medicine, Tambaram Sanatorium, Chennai, 600 047 Tamil Nadu

3. South-East Asia Regional Office, World Health Organization, New Delhi, India

Abstract

ABSTRACT We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the “gold standard.” The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantly lower than that of the standard-ZN staining method (84%; 117 of 140); the modified-ZN method missed 21% of cases detected by the standard-ZN method and 11% more of culture-positive samples than the standard-ZN method. The World Health Organization recommendation of 0.3% carbol fuchsin in the ZN method for staining AFB needs to be reconsidered.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.8.3041-3043.2002

Reference12 articles.

1. Anderson, G., and A. J. Coup. 1975. Effect of decalcifying agents on the staining of Mycobacterium tuberculosis. J. Clin. Pathol.28:744-745.

2. Central TB Division Directorate General of Health Services Ministry of Health and Family Welfare. 1998. Revised National Tuberculosis Control Programme manual for laboratory technicians. Ministry of Health and Family Welfare New Delhi India. [Online.] http://www.tbcindia.org/LABMANUAL.pdf .

3. Fodor, T. 1984. Bulk staining of sputum by the Ziehl-Neelsen method. Tubercle65:123-125.

4. Hafiz S. R. C. Spencer M. Lee H. Gooch and B. I. Duerden. 1984. Rapid Ziehl-Neelsen staining by use of microwave oven. Lancet ii: 1046.

5. Karin, W. 1998. Laboratory services in tuberculosis control. Part II. Microscopy. WHO Tech. Bull.98:258.

Cited by 17 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Odun Çeliklerinin Mikroskobik İnceleme ve Görüntülenmesinde Farklı Boyama Tekniklerinin Kullanımı Üzerine Araştırmalar;ÇOMÜ Ziraat Fakültesi Dergisi;2024-07-22

2. Evolution of Laboratory Diagnosis of Tuberculosis;Clinics and Practice;2024-02-23

3. Efficient Removal of Basic Fuchsin from Synthetic Medical Wastewater and Competitive Adsorption in the Mixture;Adsorption Science & Technology;2023-01-17

4. The Re-Identification of Previously Unidentifiable Clinical Non-Tuberculous Mycobacterial Isolates Shows Great Species Diversity and the Presence of Other Acid-Fast Genera;Pathogens;2022-10-07

5. Presence of Mycobacterium sp in the feces of captive wild birds in three municipalities of the State of Jalisco;Journal of the Selva Andina Animal Science;2021-04-01