Inefficiency of 0.3% Carbol Fuchsin in Ziehl-Neelsen Staining for Detecting Acid-Fast Bacilli-Reference-Cited by-同舟云学术

Inefficiency of 0.3% Carbol Fuchsin in Ziehl-Neelsen Staining for Detecting Acid-Fast Bacilli

Published:2002-08 Issue:8 Volume:40 Page:3041-3043
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Selvakumar N.¹,Rahman Fathima¹,Rajasekaran S.²,Narayanan P. R.¹,Frieden Thomas R.³

Affiliation:

1. Tuberculosis Research Centre, Indian Council of Medical Research, Chennai, 600 031 Tamil Nadu

2. Government Hospital for Thoracic Medicine, Tambaram Sanatorium, Chennai, 600 047 Tamil Nadu

3. South-East Asia Regional Office, World Health Organization, New Delhi, India

Abstract

ABSTRACT We compared the sensitivity and specificity of a modified Ziehl-Neelsen (modified-ZN) staining method for acid-fast bacilli (AFB) with that of the standard Ziehl-Neelsen (standard-ZN) staining method, using culture results with Mycobacterium tuberculosis as the “gold standard.” The sensitivity (72%; 101 of 140) of the modified-ZN staining method, which uses 0.3% carbol fuchsin, was significantly lower than that of the standard-ZN staining method (84%; 117 of 140); the modified-ZN method missed 21% of cases detected by the standard-ZN method and 11% more of culture-positive samples than the standard-ZN method. The World Health Organization recommendation of 0.3% carbol fuchsin in the ZN method for staining AFB needs to be reconsidered.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.8.3041-3043.2002

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