Differentiation of Dextran-Producing Leuconostoc Strains by a Modified Randomly Amplified Polymorphic DNA Protocol-Reference-Cited by-同舟云学术

Differentiation of Dextran-Producing Leuconostoc Strains by a Modified Randomly Amplified Polymorphic DNA Protocol

Published:1998-08 Issue:8 Volume:64 Page:3096-3098
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Holt Scott M.¹,Cote Gregory L.²

Affiliation:

1. Department of Biological Sciences, Western Illinois University, Macomb,1 and

2. Biopolymer Research Unit, National Center for Agricultural Utilization Research, USDA Agricultural Research Service, Peoria,2 Illinois

Abstract

ABSTRACT Seven dextran-producing Leuconostoc strains were differentiated by using a modified randomly amplified polymorphic DNA (RAPD) protocol that incorporated specific primers designed from conserved regions of dextransucrase genes. RAPD profiles showed intraspecies differences among the Leuconostoc mesenteroides strains tested. This modified RAPD protocol will aid in the differentiation of polymer-producing leuconostocs, which are currently distinguished by time-consuming analyses of the dextrans they synthesize.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.8.3096-3098.1998

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