Improvement of an Unusual Twin-Arginine Transporter Leader Peptide by a Codon-Based Randomization Approach-Reference-Cited by-同舟云学术

Improvement of an Unusual Twin-Arginine Transporter Leader Peptide by a Codon-Based Randomization Approach

Published:2006-05 Issue:5 Volume:72 Page:3797-3801
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Monroy-Lagos Olga¹,Soberon Xavier¹,Gaytan Paul¹,Osuna Joel¹

Affiliation:

1. Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología/ Universidad Nacional Autónoma de México, Cuernavaca, México

Abstract

ABSTRACT Secretion of Escherichia coli penicillin acylase was improved by codon-based random mutagenesis of its signal peptide. The mutagenesis technology was applied to the gene region coding for positions Lys2 to Thr13 (N half) and Ala14 to Leu25 (C half) of the signal peptide. Protein secretion was higher in several signal peptide variants (up to fourfold with respect to the wild-type value).

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.5.3797-3801.2006

Reference16 articles.

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