Analysis of a Gene Cluster of Enterococcus faecalis Involved in Polysaccharide Biosynthesis-Reference-Cited by-同舟云学术

Analysis of a Gene Cluster of Enterococcus faecalis Involved in Polysaccharide Biosynthesis

Published:2000-02 Issue:2 Volume:68 Page:815-823
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Xu Yi¹²,Singh Kavindra V.³²,Qin Xiang³²,Murray Barbara E.³²,Weinstock George M.¹²

Affiliation:

1. Department of Microbiology and Molecular Genetics,1

2. Center for the Study of Emerging and Re-emerging Pathogens,2 University of Texas Medical School, Houston, Texas 77030

3. Division of Infectious Diseases, Department of Medicine,3 and

Abstract

ABSTRACT Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli . The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4 , encoding a putative glycosyl transferase, and orfde6 , a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.68.2.815-823.2000

Reference27 articles.

1. The cell walls of group D streptococci. I. The immunochemistry of the type 1 carbohydrate;Bleiweis A. S.;J. Exp. Med.,1965

2. Mucoid encapsulated Enterococcus faecalis: an emerging morphotype isolated from patients with urinary tract infections;Bottone E. J.;Diagn. Microbiol. Infect. Dis.,1998

3. Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA

4. Pseudomonas aeruginosa in cystic fibrosis: role of mucC in the regulation of alginate production and stress sensitivity;Boucher J. C.;Microbiology,1997

5. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution;Chung C. T.;Proc. Natl. Acad. Sci. USA,1989

Cited by 91 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Determinants of bacterial survival and proliferation in blood;FEMS Microbiology Reviews;2024-05

2. Virulence Determinants of Enterococcus Faecium;Infectious Diseases;2024-03-27

3. The enterococci;Molecular Medical Microbiology;2024

4. Disruption of the tagF Orthologue in the epa Locus Variable Region of Enterococcus faecalis Causes Cell Surface Changes and Suppresses an eep -Dependent Lysozyme Resistance Phenotype;Journal of Bacteriology;2022-10-18

5. Antibiotics and Carbohydrate-Containing Drugs Targeting Bacterial Cell Envelopes: An Overview;Pharmaceuticals;2022-07-29