Comparison of fluorescent gentamicin-thallous-carbonate and KF streptococcal agars to enumerate enterococci and fecal streptococci in meats

Author:

Knudtson L M1,Hartman P A1

Affiliation:

1. Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.

Abstract

Two selective and differential media were compared for their abilities to enumerate enterococci and fecal streptococci in pork, beef, and poultry products. Counts obtained on KF streptococcal (KF) agar were compared with counts obtained on fluorescent gentamicin-thallous-carbonate (fGTC) agar. Reactions of 13 known enterococcal species were also observed. All 13 species of enterococci as well as Streptococcus bovis and Streptococcus equinus grew equally well on fGTC agar. KF streptococcal medium allowed growth of most species of enterococci but not S. bovis and S. equinus. Quantitative comparisons between the two media inoculated with pure cultures of known species of enterococci revealed equivalent plate counts following incubation. However, when meat samples were plated, counts on fGTC agar were consistently and significantly higher than counts on KF agar for all sample sources.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference13 articles.

1. Gentamicin-based medium for the isolation of group D streptococci and application of the medium to water analysis;Donnelly L. S.;Appl. Environ. Microbiol.,1978

2. Media and methods for isolation and enumeration of the enterococci;Hartman P. A.;Adv. Appl. Microbiol.,1966

3. Fecal streptococci. I. Cultivation and enumeration of streptococci in surface waters;Kenner B. A.;Appl. Microbiol.,1961

4. Knudtson L. M. 1992. Classification of enterococci and their roles in spoilage of pork products and as sanitary indicators in pork processing. Ph.D. thesis. Iowa State University Ames.

5. Routine procedures for isolation and identification of enterococci and fecal streptococci;Knudtson L. M.;Appl. Environ. Microbiol.,1992

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