Affiliation:
1. Swedish Institute for Food Preservation Research (SIK), Göteborg, Sweden
Abstract
Eight strains of
Clostridium botulinum
type E out of twelve tested showed good growth and normal cell morphology in a synthetic medium containing choline. Growth and toxin production by a representative strain was not influenced by repeated subculturing. In the chemically defined medium, acetylcholine,
N,N
-dimethylethanolamine, and lecithin could replace choline to get normal cell division and cell morphology of
C. botulinum
type E. Choline could not be replaced by ethanolamine,
N
-methylethanolamine, or betaine. A toxigenic strain of
C. botulinum
type E showed proteolytic, lipolytic, and lecithinase activity in complex media but not in a synthetic medium. On prolonged incubation in the high temperature range of growth, the toxicity of the culture filtrate decreased in a complex, but not in a synthetic medium. The implications of these findings are discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
5 articles.
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