Assessment of the Cavidi ExaVir Load Assay for Monitoring Plasma Viral Load in HIV-2-Infected Patients

Author:

Borrego Pedro123,Gonçalves Maria Fátima4,Gomes Perpétua34,Araújo Lavínia1,Moranguinho Inês1,Figueiredo Inês Brito13,Barahona Isabel3,Rocha José5,Mendonça Claudino5,Cruz Maria Cesarina6,Barreto Jorge7,Taveira Nuno13

Affiliation:

1. Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Lisbon, Portugal

2. Centro de Administração e Políticas Públicas, Instituto Superior de Ciências Sociais e Políticas, Universidade de Lisboa, Lisbon, Portugal

3. Centro de Investigação Interdisciplinar Egas Moniz, Instituto Superior de Ciências da Saúde Egas Moniz, Monte de Caparica, Portugal

4. Laboratório de Biologia Molecular, LMCBM, SPC, Centro Hospitalar Lisboa Ocidental—HEM, Lisbon, Portugal

5. Laboratório ELISA, Hospital Agostinho Neto, Praia, Cabo Verde

6. Laboratório de Análises Clínicas, Delegacia de Saúde, S. Vicente, Cabo Verde

7. Programa de Luta contra as Doenças de Transmissão Sexual, incluindo VIH/Sida, Ministério da Saúde e da Segurança Social, Praia, Cabo Verde

Abstract

ABSTRACT HIV plasma viral load is an established marker of disease progression and of response to antiretroviral therapy, but currently there is no commercial assay validated for the quantification of viral load in HIV-2-infected individuals. We sought to make the first clinical evaluation of Cavidi ExaVir Load (version 3) in HIV-2-infected patients. Samples were collected from a total of 102 individuals living in Cape Verde, and the HIV-2 viral load was quantified by both ExaVir Load and a reference in-house real-time quantitative PCR (qPCR) used in Portugal in 91 samples. The associations between viral load and clinical prognostic variables (CD4 + T cell counts and antiretroviral therapy status) were similar for measurements obtained using ExaVir Load and qPCR. There was no difference between the two methods in the capacity to discriminate between nonquantifiable and quantifiable HIV-2 in the plasma. In samples with an HIV-2 viral load quantifiable by both methods ( n = 27), the measurements were highly correlated (Pearson r = 0.908), but the ExaVir Load values were systematically higher relative to those determined by qPCR (median difference, 0.942 log 10 copies/ml). A regression model was derived that enables the conversion of ExaVir Load results to those that would have been obtained by the reference qPCR. In conclusion, ExaVir Load version 3 is a reliable commercial assay to measure viral load in HIV-2-infected patients and therefore a valuable alternative to the in-house assays in current use.

Funder

Luso-American Foundation for Development (FLAD), Portugal–Luso-American Collaborative Response Award on HIV/AIDS

Fundação para a Ciência e a Tecnologia, Portugal

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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