Quality Control Assessment of Human Immunodeficiency Virus Type 2 (HIV-2) Viral Load Quantification Assays: Results from an International Collaboration on HIV-2 Infection in 2006-Reference-Cited by-同舟云学术

Quality Control Assessment of Human Immunodeficiency Virus Type 2 (HIV-2) Viral Load Quantification Assays: Results from an International Collaboration on HIV-2 Infection in 2006

Published:2008-06 Issue:6 Volume:46 Page:2088-2091
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Damond Florence¹,Benard Antoine²,Ruelle Jean³,Alabi Abraham⁴,Kupfer Bernd⁵,Gomes Perpetua⁶,Rodes Berta⁷,Albert Jan⁸,Böni Jürg⁹,Garson Jeremy¹⁰,Ferns Bridget¹⁰,Matheron Sophie¹¹,Chene Geneviève²,Brun-Vezinet Françoise¹

Affiliation:

1. APHP, Hôpital Bichat—Claude Bernard, Laboratoire de Virologie, Paris, 75018 France

2. INSERM, U897.ISPED, Université Victor Segalen Bordeaux 2, Bordeaux, F-33076, France

3. AIDS Reference Laboratory, Université Catholique de Louvain, Louvain-la-Neuve, Belgium

4. Medical Research Council Laboratories, Banjul, Gambia

5. Institute of Virology, Bonn, Germany

6. Laboratório de Biologia Molecular, CHLO—Hospital Egas Moniz, Lisboa, Portugal

7. Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain

8. Department of Virology, Swedish Institute for Infectious Disease Control, Solna, and Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden

9. Swiss HIV Cohort Study, Zurich, Switzerland

10. Centre for Virology, Royal Free & University College London Medical School, London, United Kingdom

11. APHP, Hôpital Bichat—Claude Bernard, Service de Maladies Infectieuses et Tropicales, Paris, France

Abstract

ABSTRACT Human immunodeficiency virus type 2 (HIV-2) RNA quantification assays used in nine laboratories of the ACHI E V 2E (A Collaboration on HIV-2 Infection) study group were evaluated. In a blinded experimental design, laboratories quantified three series of aliquots of an HIV-2 subtype A strain, each at a different theoretical viral load. Quantification varied between laboratories, and international standardization of quantification assays is strongly needed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00126-08

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