Improved Sensitivity of Human Immunodeficiency Virus Type 2 Subtype B Plasma Viral Load Assay

Author:

Damond Florence12,Collin Gilles12,Descamps Diane12,Matheron Sophie1,Pueyo Sophie3,Taieb Audrey3,Campa Pauline4,Benard Antoine3,Chêne Geneviève3,Brun-Vezinet Francoise12

Affiliation:

1. Hôpital Bichat Claude Bernard, Paris, France

2. INSERM U552, Paris, France

3. INSERM U330, Bordeaux, France

4. Hôpital Saint Antoine, Paris, France

Abstract

ABSTRACT We developed a new assay for human immunodeficiency virus type 2 plasma RNA quantification based on a previous format. The new version performed significantly better than the original regarding the detection of subtype B, allowing the detection of 14 out of 36 plasma RNAs in the subtype B-infected patients not detected with the original version.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

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3. Berry, N., K. Ariyoshi, S. Jaffar, S. Sabally, T. Corrah, R. Tedder, and H. Whittle. 1998. Low peripheral blood viral HIV-2 RNA in individuals with high CD4 percentage differentiates HIV-2 from HIV-1 infection. J. Hum. Virol.1:457-468.

4. Human immunodeficiency virus type 2 (HIV-2) seroprevalence and characterization of a distinct HIV-2 genetic subtype from the natural range of simian immunodeficiency virus-infected sooty mangabeys

5. Damond, F., C. Apetrei, D. Descamps, F. Brun-Vezinet, and F. Simon. 1999. HIV-1 subtypes and plasma RNA quantification. AIDS13:286-288.

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