Properties of Malolactic Activity Purified from Leuconostoc oenos ML34 by Affinity Chromatography-Reference-Cited by-同舟云学术

Properties of Malolactic Activity Purified from Leuconostoc oenos ML34 by Affinity Chromatography

Published:1984-10 Issue:4 Volume:48 Page:900-901
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Spettoli Paolo¹,Nuti Marco Paolo¹,Zamorani Arturo¹

Affiliation:

1. Istituto di Chimica Agraria e Industrie Agrarie, Università di Padova, 6-35100 Padua, Italy

Abstract

Malolactic activity from Leuconostoc oenos ML34 is tightly associated with lactic dehydrogenase. A simple and fast procedure, involving affinity chromatography on agarose-hexane-NAD (Agnad), was used to separate malolactic activity from lactic dehydrogenase and other proteins. The yield was ca. 86%, the purification was 5.2-fold, and the K m values for l -malate, NAD + and Mn 2+ were 2.8, 0.13, and 0.028 mM, respectively, at a pH optimum of 5.8.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.48.4.900-901.1984

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