Author:
Chu F S,Liang M Y,Zhang G S
Abstract
Antibodies against diacetoxyscirpenol (DAS) were obtained from rabbits after immunizing them with hemisuccinate or hemiglutarate derivatives of DAS conjugated to bovine serum albumin (BSA). DAS-hemiglutarate-BSA was found to be a much better immunogen than DAS-hemisuccinate-BSA. Competitive radioimmunoassay revealed that the antisera obtained from rabbits after immunization with DAS-hemiglutarate-BSA showed high specificity toward DAS. The concentrations causing 50% displacement of radioactive DAS by unlabeled DAS, 4-monoacetoxyscirpenol (MAS), and 15-MAS were found to be 1.5, 130, and 300 ng per assay, respectively. Thus, the cross-reactivities for 4-MAS and 15-MAS are ca. 87 and 300 times weaker than that of DAS. Practically no cross-reaction (less than 5% displacement) was observed when deoxynivalenol, T-2 toxin, deoxyverrucarol, and scirpentriol were tested at a concentration of 2,000 ng/ml.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference16 articles.
1. Bamburg J. R. and F. M. Strong. 1971. 12 13-Epoxytrichothecenes p. 207-292. In S. Kadis A. Ciegler and S. Ajl (ed.) Microbial toxins vol. 7. Academic Press Inc. New York.
2. Production of antibody against T-2 toxin;Chu F. S.;Appl. Environ. Microbiol.,1979
3. Committee on Protection Against Mycotoxins. 1983. Protection against trichothecenes. National Academy Press Washington D.C.
4. Detection of T-2 toxin by an improved radioimmunoassay;Fontelo P. A.;Appl. Environ. Microbiol.,1983
5. Determination of free amino groups in proteins by trinitrobenzenesulfonic acid;Habeeb A. F. S. A.;Anal. Biochem.,1966
Cited by
30 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献