Affiliation:
1. Molecular Biology Laboratory, Research Unit, Nuestra Señora de Candelaria Hospital,1and
2. Department of Cellular Biology and Microbiology, University of La Laguna,2 Santa Cruz de Tenerife, Spain
Abstract
ABSTRACT
In this work, we describe a multiplex PCR assay for the detection of clinically relevant antibiotic resistance genes harbored by some
Staphylococcus aureus
isolates and for the simultaneous identification of such isolates at the species level. Conditions were optimized for the simultaneous detection of the 310-, 456-, and 651-bp regions of the
mecA
(encoding high-level methicillin resistance),
ileS
-
2
(encoding high-level mupirocin resistance), and
femB
(encoding a factor essential for methicillin resistance) genes, respectively, from a single colony in a single reaction tube. The
femB
PCR fragment allows the specific identification of
S
.
aureus
. Validation of the method was performed using 50 human isolates of methicillin-resistant
S
.
aureus
(MRSA) and the appropriate control strains. This assay offers a rapid, simple, feasible, specific, sensitive, and accurate identification of mupirocin-resistant MRSA clinical isolates and could be systematically applied as a diagnostic test in clinical microbiology laboratories, facilitating the design and use of antibiotic therapy.
Publisher
American Society for Microbiology
Cited by
137 articles.
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