Prevalence and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Pigs in Japan
-
Published:2024-02-04
Issue:2
Volume:13
Page:155
-
ISSN:2079-6382
-
Container-title:Antibiotics
-
language:en
-
Short-container-title:Antibiotics
Author:
Kawanishi Michiko1, Matsuda Mari1, Abo Hitoshi1, Ozawa Manao1, Hosoi Yuta1, Hiraoka Yukari1, Harada Saki1, Kumakawa Mio1, Sekiguchi Hideto1
Affiliation:
1. Veterinary AMR Center, National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo 185-8511, Japan
Abstract
We investigated the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in pig slaughterhouses from 2018 to 2022 in Japan and the isolates were examined for antimicrobial susceptibility and genetic characteristics by whole-genome analysis. Although the positive LA-MRSA rates on farms (29.6%) and samples (9.9%) in 2022 in Japan remained lower than those observed in European countries exhibiting extremely high rates of confirmed human LA-MRSA infections, these rates showed a gradually increasing trend over five years. The ST398/t034 strain was predominant, followed by ST5/t002, and differences were identified between ST398 and ST5 in terms of antimicrobial susceptibility and the resistance genes carried. Notably, LA-MRSA possessed resistance genes toward many antimicrobial classes, with 91.4% of the ST398 strains harboring zinc resistance genes. These findings indicate that the co-selection pressure associated with multidrug and zinc resistance may have contributed markedly to LA-MRSA persistence. SNP analysis revealed that ST398 and ST5 of swine origin were classified into a different cluster of MRSA from humans, showing the same ST in Japan and lacking the immune evasion genes (scn, sak, or chp). Although swine-origin LA-MRSA is currently unlikely to spread to humans and become a problem in current clinical practice, preventing its dissemination requires using antimicrobials prudently, limiting zinc utilization to the minimum required nutrient, and practicing fundamental hygiene measures.
Funder
Japanese Ministry of Health, Labor and Welfare
Subject
Pharmacology (medical),Infectious Diseases,Microbiology (medical),General Pharmacology, Toxicology and Pharmaceutics,Biochemistry,Microbiology
Reference48 articles.
1. Silva, V., Araujo, S., Monteiro, A., Eira, J., Pereira, J.E., Maltez, L., Igrejas, G., Lemsaddek, T.S., and Poeta, P. (2023). Staphylococcus aureus and MRSA in Livestock: Antimicrobial Resistance and Genetic Lineages. Microorganisms, 11. 2. Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) prevalence in humans in close contact with animals and measures to reduce on-farm colonisation;Lawlor;Ir. Vet. J.,2021 3. Staphylococcus aureus infections;Lowy;N. Engl. J. Med.,1998 4. Methicillin-resistant Staphylococcus aureus;Lee;Nat. Rev. Dis. Primers,2018 5. Fighting antibiotic resistance-strategies and (pre)clinical developments to find new antibacterials;Walesch;EMBO Rep.,2023
|
|