Affiliation:
1. Mycotoxin Research Unit, USDA/ARS, National Center for Agricultural Utilization Research, Peoria, Illinois 61604-3902
Abstract
ABSTRACT
Mutant strains of
Fusarium graminearum
Z3639 produced by disruption of
Tri8
were altered in their ability to biosynthesize 15-acetyldeoxynivalenol and instead accumulated 3,15-diacetyldeoxynivalenol, 7,8-dihydroxycalonectrin, and calonectrin.
Fusarium sporotrichioides
NRRL3299
Tri8
mutant strains accumulated 3-acetyl T-2 toxin, 3-acetyl neosolaniol, and 3,4,15-triacetoxyscirpenol rather than T-2 toxin, neosolaniol, and 4,15-diacetoxyscirpenol. The accumulation of these C-3-acetylated compounds suggests that
Tri8
encodes an esterase responsible for deacetylation at C-3. This gene function was confirmed by cell-free enzyme assays and feeding experiments with yeast expressing
Tri8.
Previous studies have shown that
Tri101
encodes a C-3 transacetylase that acts as a self-protection or resistance factor during biosynthesis and that the presence of a free C-3 hydroxyl group is a key component of
Fusarium
trichothecene phytotoxicity. Since
Tri8
encodes the esterase that removes the C-3 protecting group, it may be considered a toxicity factor.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
76 articles.
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