Comparison of the BACTEC 9240 and BacT/Alert Blood Culture Systems for Detection of Bacterial Contamination in Platelet Concentrates

Author:

Riedel Stefan1,Siwek Gregory1,Beekmann Susan E.1,Richter Sandra S.1,Raife Thomas1,Doern Gary V.1

Affiliation:

1. Divisions of Clinical Microbiology and Transfusion Medicine, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242

Abstract

ABSTRACT The BACTEC 9240 blood culture system with a standard aerobic medium, Plus Aerobic/F, was compared to the BacT/Alert system with a platelet-specific medium, BPA, as a means for detecting bacterial contamination of platelet preparations. One hundred thirteen platelet units seeded with low levels of different bacteria were examined with both systems. In 93 instances, growth was detected first in the BACTEC system; in 12 cases, growth registered first in the BacT/Alert system. Among all comparisons, growth was detected, on average, 1.7 h sooner with the BACTEC system. The differences in length of time to detection were statistically significant.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference20 articles.

1. American Association of Blood Banks. 2003. Comprehensive report on blood collection and transfusion in the United States in 2001. National Blood Data Resource Center Bethesda Md.

2. Brecher, M. E. 2002. Bacterial contamination of blood products, p. 789. In T. L. Simon (ed.), Rossi's principles of transfusion medicine, 3rd ed. Lippincott Williams and Wilkins, Baltimore, Md.

3. Bacterial Contamination of Blood Components

4. Brecher, M. E., D. G. Heath, S. N. Hay, S. J. Rothenberg, and L. C. Stutzman. 2002. Evaluation of a new generation of culture bottle using an automated bacterial culture system for detecting nine common contaminating organisms found in platelet components. Transfusion42:774-779.

5. Brecher, M. E., N. Means, C. S. Jere, D. G. Heath, S. J. Rothenberg, and L. C. Stutzman. 2001. Evaluation of an automated culture system for detecting bacterial contamination of platelets: an analysis with 15 contaminating organisms. Transfusion41:477-482.

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