Affiliation:
1. Biology Department, Rice University, Houston, Texas 77001
Abstract
A phosphorylated derivative of streptomycin accumulated in cultures of
Streptomyces griseus
ATCC 12475 and SC2376 grown on complex media containing an excess of inorganic phosphate (0.01
m
). This compound did not accumulate significantly in the absence of added inorganic phosphate. The phosphorylated derivative did not inhibit growth of
Bacillus subtilis
or support growth of a streptomycin-dependent strain of
Escherichia coli;
however, incubation of the derivative with alkaline phosphatase gave a compound which was active with both systems. In the phosphorylated derivative, phosphate is esterified with an —OH group of the streptidine moiety of streptomycin. It is suggested that the phosphoryl group is introduced during biosynthesis of the streptidine moiety of streptomycin or by the action of streptomycin kinase on preformed streptomycin (or both), and subsequent dephosphorylation by streptomycin-phosphate phosphatase is inhibited by high concentrations of inorganic phosphate. A column chromatographic procedure for separation of streptidine-phosphate, streptomycin-phosphate, and streptomycin is described.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
71 articles.
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