Use of a time-kill technique for susceptibility testing of Trichomonas vaginalis

Author:

Krieger J N,Dickins C S,Rein M F

Abstract

The emergence of metronidazole-resistant Trichomonas vaginalis and questions about the safety of metronidazole are significant concerns in treatment of trichomoniasis. At 24 h, a microtiter assay was used to test antimicrobial susceptibility of 16 recent isolates; the MICs of metronidazole ranged from less than 0.06 to 25 micrograms/ml. Observable motility as an endpoint correlated imperfectly with survival as measured in pour plates. Quantitative pour plate cultures of six T. vaginalis isolates after timed exposures to antimicrobial drugs demonstrated exquisite sensitivity to metronidazole with minimal trichomonacidal concentrations of 0.025 to 0.100 micrograms/ml. Killing of some T. vaginalis isolates by clotrimazole and rosoxacin occurred only at concentrations of 100 micrograms/ml. Resistance to both rosoxacin and clotrimazole correlated with increasing resistance to metronidazole (P less than 0.01).

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference29 articles.

1. Metronidazole metabolism in cultures of Entamoeba histolytica and Trichomonas vaginalis;Beaulieu B. B.;Antimicrob. Agents Chemother.,1980

2. Trichomonal infections of the genital tract;Catterall R. D.;Med. Clin. North Am.,1972

3. A comparison of rosoxacin with ampicillin and probenecid in the treatment of uncomplicated gonorrhea;Cohen A. I.;Sex. Transm. Dis.,1984

4. Inactivation of metronidazole by aerobic organisms;Edwards D. I.;J. Antimicrob. Chemother.,1979

5. Gibbons J. D. 1971. Nonparametric statistical inference. McGraw-Hill Book Co. New York.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3