Construction of a Xylanase-Producing Strain of Brevibacterium lactofermentum by Stable Integration of an Engineered xysA Gene from Streptomyces halstedii JM8-Reference-Cited by-同舟云学术

Construction of a Xylanase-Producing Strain of Brevibacterium lactofermentum by Stable Integration of an Engineered xysA Gene from Streptomyces halstedii JM8

Published:2001-12 Issue:12 Volume:67 Page:5425-5430
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Adham Sirin A. I.¹,Campelo Ana B.¹,Ramos Angelina¹,Gil José A.¹

Affiliation:

1. Departamento de Ecologı́a, Genética y Microbiologı́a, Área de Microbiologı́a, Facultad de Biologı́a, Universidad de León, León, Spain

Abstract

ABSTRACT A xylanolytic strain of Brevibacterium lactofermentum containing the Streptomyces halstedii His-tagged xysA gene was generated. The new strain contains DNA derived from S . halstedii , expresses xylanolytic activity, and was obtained by an integrative process mediated by a conjugative plasmid targeted to a dispensable chromosomal region located downstream from the essential cell division gene ftsZ . The His-tagged Xys1 enzyme was constitutively expressed under the control of the kan promoter from Tn 5 and was easily purified by use of Ni-nitrilotriacetic acid-agarose. The new strain is stable for more than 200 generations, lacks any known antibiotic resistance gene, and does not need any selective pressure to maintain the integrated gene. This strategy can be used to integrate any gene into the B . lactofermentum chromosome and to maintain it stably without the use of antibiotics for selection.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.12.5425-5430.2001

Reference24 articles.

1. Adham S. A. I. P. Honrubia M. Dı́az J. M. Fernández-Ábalos R. I. Santamarı́a and J. A. Gil. Expression of the genes coding for the xylanase Xys1 and the cellulase CelA 1 from the straw-decomposing Streptomyces halstedii JM8 cloned into the amino-acid producer Brevibacterium lactofermentum ATCC 13869. Arch. Microbiol. in press.

2. Enzymes of starch degradation and synthesis.;Bernfeld P.;Adv. Enzymol.,1951

3. A rapid and sensitive method for the quantification of micrograms quantities of protein utilizing the principle of protein-dye binding.;Bradford M. M.;Anal. Biochem.,1976

4. Expression of Streptomyces genes encoding extracellular enzymes in Brevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as in Streptomyces lividans.;Cadenas R. F.;Appl. Microbiol. Biotechnol.,1992

5. Construction of l-lysine-overproducing strains of Brevibacterium lactofermentum by targeted disruption of the hom and thrB genes.;Fernández-González C.;Appl. Microbiol. Biotechnol.,1996

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Role of fourteen XRE-DUF397 pairs from Streptomyces coelicolor as regulators of antibiotic production and differentiation. New players in a complex regulatory network;Frontiers in Microbiology;2023-07-10

2. Metabolic Engineering of Corynebacterium glutamicum;Metabolic Engineering;2021-06-04

3. The XRE-DUF397 Protein Pair, Scr1 and Scr2, Acts as a Strong Positive Regulator of Antibiotic Production in Streptomyces;Frontiers in Microbiology;2018-11-16

4. Industrial Microorganisms:Corynebacterium glutamicum;Industrial Biotechnology;2016-11-25

5. Recent advances in recombinant protein expression by Corynebacterium, Brevibacterium, and Streptomyces: from transcription and translation regulation to secretion pathway selection;Applied Microbiology and Biotechnology;2013-09-26