A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus-Reference-Cited by-同舟云学术

A Simple Whole-Blood Test for Detecting Antibodies to Human Immunodeficiency Virus

Published:1998-03 Issue:2 Volume:5 Page:263-265
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Schramm Willfried¹,Wade Stephen E.¹,Angulo Gustavo Barriga²,Torres Patricia Castillo²,Burgess-Cassler Anthony¹

Affiliation:

1. Saliva Diagnostic Systems, Inc., Vancouver, Washington 98682-2444,1 and

2. Clinical Laboratory, Hospital de Infectologia, Centro Medico Nacional la Raza, Instituto Mexicano del Seguro Social, Mexico City, Mexico2

Abstract

ABSTRACT We developed an immunochromatographic whole-blood test (WBT) which detects antibodies to human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) from fingerstick blood. The sensitivity and specificity of the WBT were 99.41% (1,018 confirmed positive patients) and 99.89% (941 uninfected patients), respectively (enzyme immunoassay [EIA] on serum or plasma as a reference). WBT performance was comparable to those of licensed EIAs and Western blotting, using 18 HIV-2 sera, 23 HIV-1 seroconversion panels, and a low-titer performance panel (in lieu of whole blood).

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.5.2.263-265.1998

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