Rapid Real-Time PCR Genotyping of Mutations Associated with Sulfadoxine-Pyrimethamine Resistance in Plasmodium falciparum-Reference-Cited by-同舟云学术

Rapid Real-Time PCR Genotyping of Mutations Associated with Sulfadoxine-Pyrimethamine Resistance in Plasmodium falciparum

Published:2004-08 Issue:8 Volume:48 Page:2924-2929
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Alker Alisa P.¹,Mwapasa Victor²³,Meshnick Steven R.¹⁴

Affiliation:

1. Department of Epidemiology, School of Public Health

2. Department of Community Health, College of Medicine, Blantyre, Malawi

3. Department of Epidemiology, University of Michigan, Ann Arbor, Michigan

4. Department of Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina

Abstract

ABSTRACT The resistance of Plasmodium falciparum to sulfadoxine-pyrimethamine (SP) is an emerging public health threat. Resistance to these drugs is associated with point mutations in the genes encoding dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR). We describe here an assay using real-time PCR and sequence-specific probes that detects these mutations. Using DNA from plasmids, cultured strains, and clinical samples, real-time PCR could distinguish four DHPS polymorphisms (codons 437, 540, 581, and 613) and three DHFR polymorphisms (codons 51, 59, and 108). This assay is rapid and sensitive, with a detection limit of 10 copies in most cases. This assay is amenable to large-scale studies of drug resistance.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.48.8.2924-2929.2004

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