A Rapid and Specific Genotyping Platform for Plasmodium falciparum Chloroquine Resistance via Allele-Specific PCR with a Lateral Flow Assay-Reference-Cited by-同舟云学术

A Rapid and Specific Genotyping Platform for Plasmodium falciparum Chloroquine Resistance via Allele-Specific PCR with a Lateral Flow Assay

Published:2022-04-27 Issue:2 Volume:10 Page:
ISSN:2165-0497
Container-title:Microbiology Spectrum
language:en
Short-container-title:Microbiol Spectr

Author:

Cheng Weijia¹,Song Xiaonan¹,Zhu Huiyin¹,Wu Kai²,Wang Wei³,Li Jian¹^ORCID

Affiliation:

1. School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, China

2. Department of Schistosomiasis and Endemic Diseases, Wuhan City Center for Disease Prevention and Control, Wuhan, China

3. Jiangsu Institute of Parasitic Diseases, Wuxi, China

Abstract

Accurate recognition of the mutation and genotype of genes are essential for the treatment of infectious diseases and genetic diseases. Based on the techniques of allele-specific PCR (AS-PCR) and a lateral flow assay (LFA), a rapid and useful platform for mutation detection was developed and assessed with clinical samples. It offers a powerful tool to identify antimalarial drug resistance and can support malaria control and elimination globally.

Funder

Principle Investigator Program of Hubei University of Medicine

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Cell Biology,Microbiology (medical),Genetics,General Immunology and Microbiology,Ecology,Physiology

Link

https://journals.asm.org/doi/pdf/10.1128/spectrum.02719-21

Reference29 articles.

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1. Development of methods to analyse Plasmodium falciparum single nucleotide polymorphisms in PfCRT (А > C), PfMDR1 (A > T) and PfDHFR (G > A) genes that determine resistance to quinoline, diamino-pyrimidine and sulfonamide groups of antimalarial drugs;Russian Military Medical Academy Reports;2024-04-22

2. Detection of Antimalarial Resistance-Associated Mutations in Plasmodium falciparum via a Platform of Allele-Specific PCR Combined with a Gold Nanoparticle-Based Lateral Flow Assay;Microbiology Spectrum;2022-12-21