Automatic Discrimination of Species within the Enterobacter cloacae Complex Using Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Supervised Algorithms

Author:

Candela Ana12,Guerrero-López Alejandro3,Mateos Miriam45,Gómez-Asenjo Alicia12,Arroyo Manuel J.6,Hernandez-García Marta475ORCID,del Campo Rosa475ORCID,Cercenado Emilia1289,Cuénod Aline1011,Méndez Gema6,Mancera Luis6,Caballero Juan de Dios475,Martínez-García Laura4125,Gijón Desirée475,Morosini María Isabel475,Ruiz-Garbajosa Patricia475ORCID,Egli Adrian1011,Cantón Rafael475ORCID,Muñoz Patricia1289ORCID,Rodríguez-Temporal David12ORCID,Rodríguez-Sánchez Belén12ORCID

Affiliation:

1. Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain

2. Institute of Health Research Gregorio Marañón, Madrid, Spain

3. Department of Signal Theory and Communication, University Carlos III of Madrid, Madrid, Spain

4. Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Madrid, Spain

5. Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain

6. Clover Bioanalytical Software, Granada, Spain

7. CIBER en Enfermedades Infecciosas, Madrid, Spain

8. CIBER de Enfermedades Respiratorias, CIBERES CB06/06/0058, Madrid, Spain

9. Medicine Department, Faculty of Medicine, Universidad Complutense de Madrid, Madrid, Spain

10. Applied Microbiology Research, Department of Biomedicine, University of Basel, Basel, Switzerland

11. Division of Clinical Bacteriology and Mycology, University Hospital Basel, Basel, Switzerland

12. Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública, Madrid, Spain

Abstract

The Enterobacter cloacae complex (ECC) encompasses heterogeneous clusters of species that have been associated with nosocomial outbreaks. These species may have different acquired antimicrobial resistance and virulence mechanisms, and their identification is challenging.

Funder

MEC | Instituto de Salud Carlos III

Comunidad de Madrid

Instituto de Investigación Sanitaria Gregorio Marañón

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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