Inaccuracy of Single-Target Sequencing for Discriminating Species of the Mycobacterium abscessus Group-Reference-Cited by-同舟云学术

Inaccuracy of Single-Target Sequencing for Discriminating Species of the Mycobacterium abscessus Group

Published:2009-08 Issue:8 Volume:47 Page:2596-2600
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Macheras Edouard¹²,Roux Anne-Laure³²,Ripoll Fabienne²,Sivadon-Tardy Valérie¹²,Gutierrez Cristina⁴,Gaillard Jean-Louis¹³²,Heym Beate¹²

Affiliation:

1. Laboratoire de Microbiologie, Hôpital Ambroise Paré, AP-HP, Boulogne-Billancourt

2. EA 3647, Université de Versailles Saint-Quentin-en-Yvelines, Garches

3. Laboratoire de Microbiologie, Hôpital Raymond Poincaré, AP-HP, Garches

4. INSERM 629, Institut Pasteur, Lille, France

Abstract

ABSTRACT We determined nucleotide sequences of rpoB , hsp65 , and sodA in 59 clinical isolates (from 58 patients) of the M ycobacterium abscessus group. Identification to the species level, based on three target genes, was concordant for 44 isolates (25 M. abscessus , 13 Mycobacterium massiliense , and 6 Mycobacterium bolletii isolates) and discordant for 15 isolates which had “interspecific composite patterns.” Sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00037-09

Reference37 articles.

1. Adekambi, T., P. Berger, D. Raoult, and M. Drancourt. 2006. rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov. Int. J. Syst. Evol. Microbiol.56:133-143.

2. rpoB -Based Identification of Nonpigmented and Late-Pigmenting Rapidly Growing Mycobacteria

3. Adékambi, T., and M. Drancourt. 2004. Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. Int. J. Syst. Evol. Microbiol.54:2095-2105.

4. Adékambi, T., and M. Drancourt. 2009. Mycobacterium bolletii respiratory infections. Emerg. Infect. Dis.15:302-305.

5. Amoebal Coculture of “ Mycobacterium massiliense ” sp. nov. from the Sputum of a Patient with Hemoptoic Pneumonia

Cited by 93 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Application of a new designed high resolution melting analysis for mycobacterial species identification;BMC Microbiology;2024-06-08

2. Analysis of Phenotypic and Genotypic Susceptibility to Clarithromycin and Amikacin of Mycobacterium abscessus Complex Strains Isolated from Cystic Fibrosis Patients;Microorganisms;2023-11-30

3. Mycobacterium abscessus, a complex of three fast-growing subspecies sharing virulence traits with slow-growing mycobacteria;Clinical Microbiology and Infection;2023-10

4. Identification of Mycobacterium abscessus Subspecies by MALDI-TOF Mass Spectrometry and Machine Learning;Journal of Clinical Microbiology;2023-01-26

5. Healthcare-Associated nontuberculous mycobacterial endocarditis following coronary artery angiography;International Journal of Mycobacteriology;2023