Extraction of Mycobacterium tuberculosis DNA: a Question of Containment-Reference-Cited by-同舟云学术

Extraction of Mycobacterium tuberculosis DNA: a Question of Containment

Published:2005-06 Issue:6 Volume:43 Page:2996-2997
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Somerville Wendy¹,Thibert Louise²,Schwartzman Kevin³⁴,Behr Marcel A.¹³

Affiliation:

1. McGill University Health Centre Research Institute

2. Institut National de Santé Publique du Québec

3. Department of Medicine, McGill University Health Centre

4. Respiratory Epidemiology Unit, McGill University, Montréal, Québec, Canada

Abstract

ABSTRACT DNA fingerprinting of Mycobacterium tuberculosis by IS 6110 restriction fragment length polymorphism analysis requires substantial high-quality DNA. We demonstrated that, despite extraction treatments that might be expected to inactivate this organism, M. tuberculosis remained viable during this process. These data suggest that the extraction of M. tuberculosis DNA should be performed within containment until complete.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.6.2996-2997.2005

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