Affiliation:
1. Department of Microbiology, Centre Hospitalier Universitaire, Nantes, France
Abstract
ABSTRACT
DNA fingerprinting analysis of
Mycobacterium tuberculosis
is used for epidemiological studies and the control of laboratory cross-contamination. Because standardized procedures are not entirely safe for mycobacteriology laboratory staff, the paper proposes a new technique for the processing of specimens. The technique ensures the inactivation of
M. tuberculosis
before DNA extraction without the loss of DNA integrity. The control of inactivated cultures should be rigorous and should involve the use of two different culture media incubated for at least 4 months.
Publisher
American Society for Microbiology