Perspectives and Benefits of High-Throughput Long-Read Sequencing in Microbial Ecology-Reference-Cited by-同舟云学术

Perspectives and Benefits of High-Throughput Long-Read Sequencing in Microbial Ecology

Published:2021-08-11 Issue:17 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Tedersoo Leho¹^ORCID,Albertsen Mads²,Anslan Sten¹³,Callahan Benjamin⁴

Affiliation:

1. Mycology and Microbiology Center, University of Tartu, Tartu, Estonia

2. Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark

3. Braunschweig University of Technology, Zoological Institute, Braunschweig, Germany

4. Department of Population Health and Pathobiology, College of Veterinary Medicine and Bioinformatics Research Center, North Carolina State University, Raleigh, North Carolina, USA

Abstract

Short-read, high-throughput sequencing (HTS) methods have yielded numerous important insights into microbial ecology and function. Yet, in many instances short-read HTS techniques are suboptimal, for example, by providing insufficient phylogenetic resolution or low integrity of assembled genomes. Single-molecule and synthetic long-read (SLR) HTS methods have successfully ameliorated these limitations.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00626-21

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